Results
| PMID | 21987111 |
| Gene Name | LIF |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 16 |
| Population details | 16 (8 with endometriosis, 8 fertile controls) |
| Sex | Female |
| Associated genes | HOXA11, LIF and BTEB1 |
| Other associated phenotypes |
Endometriosis |
|
Iran Biomed J. 2011;15(3):66-72. Alizadeh, Zohreh| Shokrzadeh, Naser| Saidijam, Massoud| Sanoee, Marzieh Farimani Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.| Dept. of Anatomy, Gonabad University of Medical Sciences, Gonabad, Khorasan Razavi, Iran.| Research Center for Molecular Medicine, Hamadan University of Me BACKGROUND: HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation. We also describe a semi-quantitative RT-PCR protocol optimized in our laboratory. METHODS: Eight patients with endometriosis were considered as our case and 8 fertile women as control group. Expression levels of HOXA11, LIF and BTEB1 mRNA were measured in endometrium during the mid-secretory phase using semi-quantitative RT-PCR. RESULTS: We describe the detailed procedure for the analysis of HOXA11, LIF and BTEB1 mRNA levels. Endometrial HOXA11 and LIF mRNA expression levels (normalized to beta-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls at the time of implantation (P < 0.05). A similar trend was seen in BTEB1 mRNA expression. CONCLUSION: The results suggest that the alteration in expression pattern of the some genes could account for some aspects of infertility in endometriosis. Mesh Terms: Base Sequence| Case-Control Studies| DNA Primers| Electrophoresis, Agar Gel| Endometriosis/*genetics| Endometrium/*secretion| Female| Homeodomain Proteins/*genetics| Humans| Kruppel-Like Transcription Factors/*genetics| Leukemia Inhibitory Facto |
|